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its due to overactivity of the immune system resulting in skin inflammation, Dr

RNA extraction, cDNA synthesis, and RT-qPCR Total RNA was extracted from liver tissue homogenized in TRIzol Reagent (Invitrogen), following the manufacturers protocol

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These primers were synthesized in IDT (Integrated DNA Technologies, Coralville, IA)

JK: Conceptualization, Data curation, Formal Analysis, Funding acquisition, Investigation, Methodology, Project administration, Resources, Software, Supervision, Validation, Visualization, Writing original draft, Writing review and editing

Cells were imaged using an inverted Nikon A1R HD microscope with 405, 488, and 561 nm laser lines with a CFI Apochromat TIRF 60x oil objective using Nikon NIS-Elements AR version 5.42.01 build 1793